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Cloning and characterization of a novel CoA-ligase gene fromPenicillium chrysogenum

โœ Scribed by Zhou-Liang Yu; Jing Liu; Fu-Qiang Wang; Meng Dai; Bao-Hua Zhao; Jian-Gong He; Hua Zhang


Book ID
107628371
Publisher
Springer Netherlands
Year
2011
Tongue
English
Weight
362 KB
Volume
56
Category
Article
ISSN
0015-5632

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A hybrid cefE gene was constructed by juxtaposing promoter sequences from the Penicillium chrysogenum pcbC gene to the open reading frame of the Streptomyces clavuligerus cefE gene. In S. clavuligerus the cefE gene codes for the enzyme penicillin N expandase [also known as deacetoxycephalosporin C s