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Cloning and characterization of a 70 kd heat shock cognate (hsc70) gene from the zebrafish (Danio rerio)

✍ Scribed by Robert T. Graser; Daniela Malnar-Dragojevic; Vladimir Vincek

Publisher: Springer Netherlands
Year: 1996
Tongue: English
Weight: 331 KB
Volume: 98
Category: Article
ISSN: 0016-6707
DOI: 10.1007/bf00057591

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✦ Synopsis

The heat shock 70 family of proteins is one of the most highly conserved among all species. The genes encoding these proteins have been cloned and sequenced from bacterial species to humans with a high degree of homology preserved throughout evolution. Here we describe the cloning and characterization of a cDNA encoding a 70 kd heat shock cognate (hsc70) gene from the zebrafish (Danio rerio). A high degree of conservation is observed among hsc70 genes of other species as shown by phylogenetic analysis. The characterization of a hsc70 gene in the zebrafish provides a marker for studying the role of a constitutively expressed member of the hsp70 family in an important developmental and evolutionary model system.

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