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Cloning and analysis of the MAT1-2-1 gene from the traditional Chinese medicinal fungus Ophiocordyceps sinensis

✍ Scribed by Shu Zhang; Yong-Jie Zhang; Xing-Zhong Liu; Hua-An Wen; Mu Wang; Dian-Sheng Liu


Book ID
116496346
Publisher
Elsevier
Year
2011
Tongue
English
Weight
570 KB
Volume
115
Category
Article
ISSN
1878-6146

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The gene INV1 encoding invertase from the yeast Candida utilis has been cloned using a homologous PCR hybridization probe, amplified with two sets of degenerate primers designed considering sequence comparisons between yeast invertases. The cloned gene was sequenced and found to encode a polypeptide