Clonally expanded Vα12+ (AV12S1),CD8+ T cells from a patient with rheumatoid arthritis are autoreactive
✍ Scribed by Samuel M. Behar; Chris Roy; James Lederer; Pat Fraser; Michael B. Brenner
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 889 KB
- Volume
- 41
- Category
- Article
- ISSN
- 0004-3591
No coin nor oath required. For personal study only.
✦ Synopsis
Objective. Previously, we showed that 15-20% of patients with rheumatoid arthritis (RA) have oligoclonal expansions of peripheral blood CDS+ T cells expressing T cell receptors encoded by the VJ2 (AV12S1) gene. To better understand the significance of these expansions, the present study was undertaken to determine their specificity.
Methods. We cloned and characterized V,12+,CDS+ T cells from the peripheral blood of 1 RA patient with a clonal expansion of these T cells.
Results. The T cell clones were autoreactive since they recognized autologous, but not allogeneic, antigenpresenting cells. Upon activation, these T cells secreted interleukin-4 and interleukin-10. The autoreactive T cell clones were class I major histocompatibility complex (MHC) restricted, by either HLA-B60 or HLA-Cw3.
Conclusion. A large population of class I MHCrestricted CDS+ T cells in a patient with RA is clonally expanded and autoreactive. These cells define a novel
immune aberration in RA and provide a tool for defining the autoantigens that activate expanded T cell populations in vivo.
Since the original report from this laboratory of clonal and oligoclonal expansions of V,12+ (AV12Sl),CD8+ T cells in the blood and synovial tissue of patients with rheumatoid arthritis (RA) (l), subsequent reports have documented expansions of CD8+ T cells in both normal individuals and patients with a