✦ LIBER ✦

Clonal propagation ofAcacia salignaby shoot tip culture

✍ Scribed by M. N. Barakat; M. H. El-Lakany

Publisher: Springer
Year: 1992
Tongue: English
Weight: 325 KB
Volume: 59
Category: Article
ISSN: 0014-2336
DOI: 10.1007/bf00041260

No coin nor oath required. For personal study only.

✦ Synopsis

Multiple shoots buds were obtained successfully from shoot tips of Acacia saligna by placing explants into solidified Murashighe & Skoog (1962) medium (MS medium) supplemented with 5.0 to 9.0 mg/L BAP. Sequential culture treatment was highly effective for shoot elongation using MS medium containing 0.3 mg/L BAP and 0.2 mg/L IAA. The shoots rooted best on MS medium supplemented with 2.0 mg/L IBA. Plantlet survival after transfer to soil was more than 90%. The shoot proliferation method described could be used for the mass clonal propagation of selected genotypes.

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