Clonal propagation of rice through proliferation of axillary shoots

✍ Scribed by B. Greco; A. Lomonaco; B. Boggini; C. Tomassini; O. A. Tanzarella

• Publisher: Springer
• Year: 1990
• Tongue: English
• Weight: 355 KB
• Volume: 48
• Category: Article
• ISSN: 0014-2336
• DOI: 10.1007/bf00037191

✦ Synopsis

Shoot base segments have been explanted from seedlings of rice (Oryza sativa L . subsp . Japonica, cv .

Arborio) and grown on agar-solidified MS medium supplemented with different concentrations of four cytokinins : kinetin, BAP, 2iP and zeatin . After one month, segments were explanted from proliferated shoots and subcultured on their respective media . BAP was by far the most effective in inducing shoot proliferation . Highest rates were achieved at the higher concentration used : 5 mg 1 -1 . Shoot base segments were subcultured fifteen times consecutively on seven different concentrations of BAP . Shoots grown in the presence of 5 mg 1 -1 of BAP proliferated an average of 12 normal shoots for each base segment throughout the fifteen subcultures . The shoots rooted easily on hormone-free medium . The technique does not require any particular skill, it is very effective and, therefore, can be suggested as suitable for clonal propagation of rice .