𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Clonal growth of cells taken directly from adult rabbits

✍ Scribed by Richard G. Ham; Louann W. Murray

Publisher
John Wiley and Sons
Year
1967
Tongue
English
Weight
563 KB
Volume
70
Category
Article
ISSN
0021-9541

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✦ Synopsis

Single cell suspensions were prepared from the choroids of adult rabbit eyes using Pronase digestion, gentle pipetting and centrifugation. When diluted and inoculated into medium F12 supplemented with 5% fetal bovine serum and 10% rabbit serum, the choroid cell suspensions yielded large, healthy colonies with an average plating efficiency of about 45%. The growth in culture of a large portion of the cells from a normal non-embryonic tissue opens new possibilities for the study of cellular differentiation i n v i h o .

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