Clonal growth of bladder cancer cells in a double layer soft agar assay: needles addition of multiple culture supplements
✍ Scribed by Lee, K. ;Smith, P. ;Weiss, G. H. ;Cockett, A. T. K.
- Book ID
- 104709921
- Publisher
- Springer
- Year
- 1990
- Tongue
- English
- Weight
- 416 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0300-5623
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✦ Synopsis
The necessity of multiple culture supplements, characteristic of Hamburger and Salmon (H&S) soft agar assay (1977), for the clonal growth of bladder cancer cells was systematically investigated. One murine and 6 human cell lines were cultivated in different culture supplemented conditions based on the H&S method. All cell lines successfully formed colonies in the absence of supplements. The inclusion of 2-mercaptoethanol to supplement-free medium stimulated the clonal growth of murine (MBT-2) cells, whereas it did not affect the growth of human cell lines. DEAE-dextran suppressed the clonal growth of 5 out of 7 lines. Tryptic soy broth stimulated the growth of 2 human cell lines, whereas it suppressed the other 2 cell lines. The chemosensitivity of MBT-2 cells was not affected by these supplements.