𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Clonal analysis of the action of GM-CSF on the proliferation and differentiation of myelomonocytic leukemic cells

✍ Scribed by D. Metcalf

Book ID
102863524
Publisher
John Wiley and Sons
Year
1979
Tongue
French
Weight
734 KB
Volume
24
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

With 214 subclones of the BALB/c myelomonocytic leukemia WEHI‐3B, the granulocyte‐macrophage colony‐stimulating factor (GM‐CSF) in impure or purified form, consistently increased the proportion of colonies exhibiting partial or complete differentiation in agar cultures. GM‐CSF also increased colony size and content of daughter colony‐forming cells. Serial recloning of WEHI‐3B colonies in the presence of GM‐CSF showed that when colonies differentiated completely, self‐replication of the colony‐forming cell was suppressed (clonal extinction). However, WEHI‐3B cells exhibited clonal instability and even in the continuous presence of GM‐CSF many colony‐forming cells still generated cells able to form undifferentiated colonies. It appears unlikely that GM‐CSF can completely suppress the progressive proliferation of a myeloid leukemic population of the WEHI‐3B type.

📜 SIMILAR VOLUMES

Characterization of a serum factor stimu
Characterization of a serum factor stimulating the differentiation of myelomonocytic leukemic cells
✍ Antony W. Burgess; Donald Metcalf 📂 Article 📅 1980 🏛 John Wiley and Sons 🌐 French ⚖ 815 KB

## Abstract Culture of WEHL‐3B myelomonocytic leukemic cells in semi‐solid agar medium containing serum from mice injected with endotoxin serum (ES) led to the development of maturing granulocytes and macrophages in most leukemic colonies. ES contains high levels of granulocyte‐macrophage colony‐st

Autocrine activation of the IL-3/GM-CSF/
Autocrine activation of the IL-3/GM-CSF/IL-5 signaling pathway in leukemic cells
✍ Paul, Cassandra C.; Mahrer, Susan; McMannama, Karyle; Baumann, Michael A. 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 454 KB 👁 2 views

The AML14.3D10 human myeloid leukemic cell line expresses receptors for granulocytemacrophage colony stimulating factor (GM-CSF) and interleukin-5 (IL-5), but not IL-3. We have found that this cell line produces GM-CSF in amounts up to 113 pg/ml in culture supernatants. Deprivation of endogenous GM-