Clinically tolerable concentrations of arsenic trioxide induce p53-independent cell death and repress NF-κB activation in Ewing sarcoma cells
✍ Scribed by Julie Mathieu; Françoise Besançon
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- French
- Weight
- 324 KB
- Volume
- 119
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
Abstract
Ewing sarcoma (ES), a highly malignant pediatric tumor, is consistently associated with translocations that fuse the EWS gene with a member of the ETS family gene, most commonly FLI‐1. Despite significant advances with multiagent chemotherapy, surgery and radiotherapy, about 40% of ES patients still die from the disease. It is therefore necessary to explore novel agents for possible treatment of this tumor. Here the authors investigated the sensitivity of ES cells to clinically tolerable concentrations of arsenic trioxide (As~2~O~3~), a compound known to induce differentiation and apoptosis of other types of malignant cells. The authors report that As~2~O~3~ uniformly induced death of 6 ES‐derived cell lines irrespective of their p53 status. As~2~O~3~ resulted in an apoptotic phenotype which was inhibited by the broad‐spectrum caspase inhibitor ZVAD‐fmk. These effects correlated with prolonged c‐jun N‐terminal kinase activation, which is a signal for apoptosis in ES cells. As~2~O~3~ also decreased basal and cytokine‐induced NF‐κB activity. Since the authors previously demonstrated that NF‐κB exerts an antiapoptotic action in ES cells, As~2~O~3~ treatment may also result in a sensitization of these cells to other drugs used in combination therapy. These effects, combined with its antiangiogenic action, define As~2~O~3~ as a good candidate for future protocols to improve treatments of Ewing sarcomas, irrespective of the p53 status of the tumor. © 2006 Wiley‐Liss, Inc.