✦ LIBER ✦

Clinical applications of luminescent assays for enzymes and enzyme labels

✍ Scribed by Irena Bronstein; Larry J. Kricka

Publisher: John Wiley and Sons
Year: 1989
Tongue: English
Weight: 642 KB
Volume: 3
Category: Article
ISSN: 0887-8013
DOI: 10.1002/jcla.1860030511

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✦ Synopsis

A wide range of luminescent assays for enzyme labels has been developed. The clinical application of luminescent assays for horseradish peroxidase, alkaline phosphatase, xanthine oxidase, beta-D galactosidase, and glucose 6-phosphate dehydrogenase is discussed. The enzyme luminescent methods provide improved sensitivity compared with colorimetric and fluorimetric assays. A new reagent, dioxetane phosphate, has provided a simple and extremely sensitive assay for alkaline phosphatase. The available instruments and new photographic detection techniques for luminescent assays are briefly discussed.

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