✦ LIBER ✦

Clinical application of K-ras oncogene mutations in pancreatic carcinoma: Detection of micrometastases

✍ Scribed by Shuji Nomoto; Akimasa Nakao; Nobuhisa Ando; Shin Takeda; Yasushi Kasai; Soichiro Inoue; Tetsuya Kaneko; Hiroshi Takagi

Publisher: John Wiley and Sons
Year: 1998
Tongue: English
Weight: 46 KB
Volume: 15
Category: Article
ISSN: 8756-0437
DOI: 10.1002/(sici)1098-2388(199807/08)15:1<40::aid-ssu7>3.0.co;2-w

No coin nor oath required. For personal study only.

✦ Synopsis

Pancreatic adenocarcinomas are known to have a high incidence of K-ras gene mutation. We summarize our efforts to detect micrometastases through a search for mutated K-ras oncogene in liver tissues, peritoneal washings, para-aortic lymph nodes, and perioperative peripheral blood. Two-stage polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis were used to detect K-ras oncogene mutations at codon 12. Our results suggest that PCR/RFLP analysis is potentially highly sensitive for the detection of micrometastases in various tissues and might be of value in the diagnosis, treatment and follow-up of metastases in other organs with pancreatic adenocarcinoma.

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