Clinical applicability of single-tube multiplex reverse-transcriptase PCR in dengue virus diagnosis and serotyping

✍ Scribed by Bajayantimala Mishra; Mirnalini Sharma; Sujit Kumar Pujhari; Suma B. Appannanavar; Radha Kanta Ratho

• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 76 KB
• Volume: 25
• Category: Article
• ISSN: 0887-8013
• DOI: 10.1002/jcla.20434

✦ Synopsis

Abstract

This study has evaluated the clinical applicability of a single‐tube multiplex RT‐ PCR as compared with a two‐step nested RT‐PCR for the diagnosis as well as serotyping of dengue virus in patient's samples. Seventy‐six acute phase blood samples collected from clinically suspected dengue patients during the 2008 outbreak were subjected to two‐step nested RT‐PCR and single‐tube multiplex RT‐PCR for dengue diagnosis and serotyping. Of the 76 samples, 17 (22.4%) were positive for dengue viral RNA. Single dengue virus infection was found in 16 cases and 1 had concurrent infection with two serotypes (3&1). Dengue serotype 3 was the predominant serotype (70.5%), followed by serotype 1 (23.5%). Single‐tube multiplex PCR had concordant result with that of two‐step nested RT‐PCR including the one with concomitant infection. This study reveals the predominance of dengue serotype 3 in North India in addition to the co‐circulation of multiple serotypes and concomitant infection. The rapid and accurate diagnostic capability of single‐tube multiplex RT‐PCR used in the study appears to be promising enough to be commonly used for dengue viral detection as well as serotyping. J. Clin. Lab. Anal. 25:76–78, 2011. © 2011 Wiley‐Liss, Inc.