Clinical and analytical performance of automated immunochemiluminometric assays for determination of cardiac markers in serum

✍ Scribed by Panteghini, Mauro

• Publisher: John Wiley and Sons
• Year: 1998
• Weight: 36 KB
• Volume: 13
• Category: Article
• ISSN: 0884-3996
• DOI: 10.1002/(sici)1099-1271(1998090)13:5<307::aid-bio490>3.0.co;2-4

✦ Synopsis

Three new immunochemiluminometric assays for quantitation of cardiac markers, i.e. creatine kinase isoenzyme MB (CK-MB), myoglobin and cardiac troponin I (cTnI), were evaluated with the Sano® Access analyser. The complete pro®le requires 20 min to perform, the method being suitable in true stat situations. In patients with early myocardial infarction (median time of sample collection: 210 min from onset, range 30±450; n = 44), the diagnostic sensitivity of Access cTnI was 66%, compared with 80% for myoglobin, and 43% for CK-MB. For comparison, cTnI, with an automated immuno¯uorimetric assay was also measured (sensitivity, 45%; p `0.05 vs. Access cTnI). Our data con®rmed myoglobin as the ®rst biochemical marker to appear elevated after infarction. However, cTnI may be a more sensitive marker for early detection of cardiac damage than initially thought, when determined by an ultrasensitive method such as an immunochemiluminometric assay.