Cleaving of S-mandelonitrile catalyzed by S-hydroxynitrile lyase from Hevea brasiliensis—a kinetic investigation based on the rate curve method

Kinetic studies on the enzyme (S)-hydrox

Kinetic studies on the enzyme (S)-hydroxynitrile lyase from Hevea brasiliensis using initial rate methods and progress curve analysis

✍ Michael Bauer; Herfried Griengl; Walter Steiner 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 292 KB

S)-Hydroxynitrile lyase (EC 4.1.2.39) from Hevea brasiliensis (rubber tree) catalyzes the reversible cleavage of cyanohydrins to aldehydes or ketones and prussic acid (HCN). Enzyme kinetics in both directions was studied on a model system with mandelonitrile, benzaldehyde, and HCN using two differen