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Cleavage of γ-carboxyglutamyl peptide bonds by cyanogen bromide and by N-bromosuccinimide

✍ Scribed by Kouichi Katayama; Koiti Titani

Book ID
115908252
Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
289 KB
Volume
95
Category
Article
ISSN
0014-5793

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Enhancement of Cyanogen Bromide Cleavage
Enhancement of Cyanogen Bromide Cleavage Yields for Methionyl-Serine and Methionyl-Threonine Peptide Bonds
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Cyanogen bromide (CNBr) is a common chemical used to hydrolyze peptide bonds C-terminal to methionine residues in peptides and proteins. In most cases, the efficiency of this bond cleavage is greater than 90% except in situations where a serine or threonine residue follows methionine in the amino ac