Cleavage of bacteriophage M13 DNA byHaemophilus influenzaeendonuclease-R

✍ Scribed by C. M. Hondel; J. G. G. Schoenmakers

• Book ID: 104738499
• Publisher: Springer
• Year: 1973
• Tongue: English
• Weight: 300 KB
• Volume: 1
• Category: Article
• ISSN: 0301-4851
• DOI: 10.1007/bf00357404

✦ Synopsis

The restriction enzyme from Haemophilus influenzae, endonuclease-R, has only one cleavage site on the double-stranded replicative form DNA of bacteriophage M13. Circular replicative forms are broken to yield full-length liniar M 13-DNA molecules (RF-III). The cleavage site appears to be specific as the RF-III molecules, produced by endonuclease-R, cannot be circularized by denaturation and renaturation.