## Abstract Mature unfertilized eggs of the medaka, __Oryzias latipes__, were injected with sperm flagellar microtubules of the sea urchin, __Hemicentrotus pulcherrimus__, and were observed with reference to the process of early development. Groups of injected eggs, most of which initiated cleavage
Cleavage initiating activities of sperm fractions injected into the egg of the medaka,Oryzias latipes
✍ Scribed by Iwamatsu, T. ;Ohta, T.
- Publisher
- John Wiley and Sons
- Year
- 1974
- Tongue
- English
- Weight
- 866 KB
- Volume
- 187
- Category
- Article
- ISSN
- 0022-104X
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✦ Synopsis
Abstract
Fractions of sperm homogenates from various animals (medaka, loach, toad, and rat) have been injected into unfertilized mature eggs of the medaka, Oryzias latipes, to test their cleavage‐initiating activity (CIA). The relationship between CIA and components of each fraction was also examined by observation with an electron microscope. It was found that there was high CIA in the fraction containing flagella or microtubules, but little CIA in the supernatant fluid without them. The CIA was closely related to the concentration of flagellum components and had no species‐specificity. However, further development after the blastula stage might be influenced by a species‐specific factor(s) of some flagellum components.
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