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Cleavage initiating activities of sperm fractions injected into the egg of the medaka,Oryzias latipes

✍ Scribed by Iwamatsu, T. ;Ohta, T.


Publisher
John Wiley and Sons
Year
1974
Tongue
English
Weight
866 KB
Volume
187
Category
Article
ISSN
0022-104X

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✦ Synopsis


Abstract

Fractions of sperm homogenates from various animals (medaka, loach, toad, and rat) have been injected into unfertilized mature eggs of the medaka, Oryzias latipes, to test their cleavage‐initiating activity (CIA). The relationship between CIA and components of each fraction was also examined by observation with an electron microscope. It was found that there was high CIA in the fraction containing flagella or microtubules, but little CIA in the supernatant fluid without them. The CIA was closely related to the concentration of flagellum components and had no species‐specificity. However, further development after the blastula stage might be influenced by a species‐specific factor(s) of some flagellum components.


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Morphological observations on cleavage o
✍ Ohta, Tadayuki ;Iwamatsu, Takashi 📂 Article 📅 1981 🏛 John Wiley and Sons 🌐 English ⚖ 880 KB

## Abstract Mature unfertilized eggs of the medaka, __Oryzias latipes__, were injected with sperm flagellar microtubules of the sea urchin, __Hemicentrotus pulcherrimus__, and were observed with reference to the process of early development. Groups of injected eggs, most of which initiated cleavage