FlowMetrix (Luminex, Austin, TX) microspheres were recently introduced as a platform for beadbased assays involving antibodies, enzymes, toxins, and nucleic acids. The procedure involves classification of the microspheres by their orange and red fluorescence and quantitation of the BODIPY-tagged bio
Classification accuracy in multiple color fluorescence imaging microscopy
โ Scribed by Kenneth R. Castleman; R. Eils; Larry Morrison; Jim Piper; Kaan Saracoglu; Mark A. Schulze; Michael R. Speiche
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 369 KB
- Volume
- 41
- Category
- Article
- ISSN
- 0196-4763
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Methods for numerical description and subsequent classification of cellular protein localization patterns are described. Images representing the localization patterns of 4 proteins and DNA were obtained using fluorescence microscopy and divided into distinct training and test sets. The images were p
The fact that ncarly all humans have their heart on the left hand side is taken for granted by most people, without appreciating the complexity of the explanations needed to account for why this is consistently so. In many organisms we can understand, in principle, how the antero-posterior, and dorw
In the myelodysplastic syndrome (MDS), cytogenetic abnormalities are often present and can be used as markers in studies for cell lineage involvement. Little is known of the involvement of the megakaryocytic lineage due to the variable ploidy of these cells. We applied dual-color fluorescence in sit