Circularly polarized Raman spectroscopy: Direct determination of antisymmetric scattering in the resonance Raman spectrum of ferrocytochrome c

✍ Scribed by James Nestro; Thomas G. Spiro

• Publisher: John Wiley and Sons
• Year: 1973
• Tongue: English
• Weight: 591 KB
• Volume: 1
• Category: Article
• ISSN: 0377-0486
• DOI: 10.1002/jrs.1250010604

✦ Synopsis

Abstract

An apparatus is described for measuring Raman backscattering with both linearly and circularly polarized light. All four polarization components, parallel, perpendicular, corotating and contrarotating, can be directly compared and used to determine the relative magnitudes of the three invariants of the non‐symmetric Raman tensor for randomly oriented molecules. The method has been applied to the resonance Raman spectrum of ferrocytochrome c. Antisymmetric scattering, previously detected through anomalous depolarization ratios, has been confirmed by direct measurement of the antisymmetric tensor invariant. In addition to the four anomalously polarized bands, two bands with normal polarizations have been shown to exhibit significant antisymmetric scattering. This result implies a reduction from four‐hold symmetry for the heme chromosphere.