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Circular mitochondrial DNA from Xenopus laevis and Rana pipiens

✍ Scribed by David R. Wolstenholme; Igor B. Dawid

Book ID
104754193
Publisher
Springer
Year
1967
Tongue
English
Weight
902 KB
Volume
20
Category
Article
ISSN
0009-5915

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✦ Synopsis

Mitochondrial DNA from oocytes of Xenopus laevis and Rana pipiens and from the liver of Gallus domesticus was studied by electron microscopy using the Kleinschmidt technique. A high percentage of circular molecules, either highly twisted or open, was observed in all preparations. The mean contour length of circles from X. laevis was 5.40 ~, from R. pipiens 5.56 ~ and from G. domesticus 5.26 ~z.

Highly twisted circles were found in greater abundance in a fresh preparation than in preparations left standing for 3 months. These molecules are considered to be the native form of mitochondrial DNA.

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