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Circular dichroism spectroscopy of fluorescent proteins

✍ Scribed by Nina V Visser; Mark A Hink; Jan Willem Borst; Gerard N.M van der Krogt; Antonie J.W.G Visser


Book ID
117104265
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
168 KB
Volume
521
Category
Article
ISSN
0014-5793

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We compared changes in the fluorescence, circular dichroism (CD) and multiply charged electrospray ionization mass spectrometry (ESI-MS) spectra of three calcium (Ca 2 )-binding proteins upon the binding of Ca . The proteins used were rat brain calbindin D 28K and two deletion mutants, one lacking E