𝔖 Bobbio Scriptorium
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Circular dichroism of DNA: Temperature and salt dependence

✍ Scribed by David S. Studdert; Maria Patroni; Robert C. Davis

Publisher
Wiley (John Wiley & Sons)
Year
1972
Tongue
English
Weight
926 KB
Volume
11
Category
Article
ISSN
0006-3525

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✦ Synopsis

The temperature dependence of the circular dichroism and ultraviolet absorbance of calf thymus DNA and poly dAT were measured in aqueous solutions of various 1-1 electrolytes. Although there was very little change in absorbance, the circular dichroism changed dramatically as a function of both temperature and solvent. There is a correlation of the heat of hydration of the cation and the magnitude of the temperature dependence of the circular dichroism. Our results are interpreted in terms of a large number (possibly a continuum) of intermediate secondary structures.

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