Circular dichroism of biological membranes-brain microsomes

✍ Scribed by Long, Marianna M. ;Masotti, Lanfranco ;Sachs, George ;Urry, Dan W.

• Book ID: 102438354
• Publisher: Wiley (John Wiley & Sons)
• Year: 1973
• Tongue: English
• Weight: 425 KB
• Volume: 1
• Category: Article
• ISSN: 0091-7419
• DOI: 10.1002/jss.400010403

✦ Synopsis

Abstract

The circular dichroism and absorption spectra of particulate and solubilized brain microsomes are reported. The corrected molar ellipticity for the membrane suspension was −1.1 × 10^+4^, between the values of axonal and oxyntic cell membranes and those for sarcotubular vesicles. No conformational change was detected which correlated unambiguously with the active cation transport function of these microsomes. Divalent cations, however, elicited a significant change in the membranes' ultra structure. Correction of suspension data for the NaI microsomes with and without sonication gave virtually identical values providing optimism for the corrections used and validating the difference in corrected values for ths suspension with and without magnesium. This indicates that, in addition to causing a change in the state of aggregation, 2 mM magnesium ion may reasonably be considered to effect a change in protein conformation.