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Circular dichroism evidence for G·U and G·T base pairing in poly[r(G-U)] and poly[d(G-T)]

✍ Scribed by Donald M. Gray; Robert L. Ratliff

Publisher
Wiley (John Wiley & Sons)
Year
1977
Tongue
English
Weight
575 KB
Volume
16
Category
Article
ISSN
0006-3525

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✦ Synopsis

Abstract

We have measured the circular dichroism (CD) and absorption properties of poly[r(G‐U)] and poly [d(G‐T)] over a wide range of Na^+^ concentrations and temperatures. We find evidence for self‐complexed forms of these polymers at lower temperatures and/or higher Na^+^ concentrations than generally needed for double‐strand formation in other DNA and RNA polymers. These self‐complexes could be composed of double‐stranded regions with weak G·U or G·T base pairs.

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Circular dichroism spectra of poly[d(AC)
Circular dichroism spectra of poly[d(AC):d(GT)], poly[r(AC):r(GU)], and hybrids poly[d(AC):r(GU)] and poly[r(AC):d(GT)] in the presence of ethanol
✍ Donald M. Gray; Robert L. Ratliff 📂 Article 📅 1975 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 691 KB

## Abstract Ultraviolet circular dichroism spectra have been obtained in aqueous solutions in the presence and absence of ethanol for a synthetic DNA, poly[d(AC):d(GT)], a synthetic RNA, poly[r(AC):r(GU)], and two DNA:RNA hybrids, poly[d(AC):r(GU)] and poly[r(AC):d(GT)]. In the absence of ethanol,