𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Circular dichroism as a detection method in the screening of enantioselective catalysts

✍ Scribed by Manfred T. Reetz; Klaus M. Kühling; Heike Hinrichs; Alfred Deege

Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
95 KB
Volume
12
Category
Article
ISSN
0899-0042

No coin nor oath required. For personal study only.

✦ Synopsis

The combination of liquid chromatography (HPLC), UV/Vis-spectroscopy and circular dichroism (CD) can be used to construct a high-throughput screening system to determine the enantioselectivity of enzyme- or metal-catalyzed reduction of acetophenone with formation of (S)- and (R)-1-phenylethanol. Prerequisite for the viability of this system is the experimental finding that the anisotropy factor g is linearly related to the enantiomeric excess (ee) and that it is independent of concentration, thereby excluding possible aggregation effects.

📜 SIMILAR VOLUMES

Development of a nonchiral HPLC method w
Development of a nonchiral HPLC method with circular dichroism detection for chiral analysis of molybdenum-catalyzed enantioselective synthesis products
✍ Michael T. Miller; Zhihong Ge; Bing Mao 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 90 KB 👁 1 views

## Abstract The combination of a circular dichroism detector and nonchiral liquid chromatography was used for the chiral analysis of unresolved enantiomers to determine the enantioselectivity of a molybdenum‐catalyzed asymmetric allylic alkylation reaction. The CD/UV peak area ratio of the unresolv

Unconventional method based on circular
Unconventional method based on circular dichroism to detect peanut DNA in food by means of a PNA probe and a cyanine dye
✍ Stefano Sforza; Elena Scaravelli; Roberto Corradini; Rosangela Marchelli 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 187 KB 👁 2 views

## Abstract In this paper we report an innovative and unconventional method based on circular dichroism for the identification of peanut DNA in food, which can be detected after PCR amplification at the nanomolar level by using an achiral PNA probe complementary to a tract of the peanut Ara h 2 gen

A fast screening MALDI method for the de
A fast screening MALDI method for the detection of cocaine and its metabolites in hair
✍ Susanna Vogliardi; Donata Favretto; Giampietro Frison; Santo Davide Ferrara; Rob 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 175 KB

## Abstract Matrix‐assisted laser desorption/ionisation (MALDI) mass spectrometry was used for the rapid detection of cocaine, benzoylecgonine and cocaethylene in hair. Different MALDI sample preparation procedures have been tested and the employment of a multi‐layer ‘graphite–sample–electrosprayed