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Circular dichroism and nucleotide and phosphate-induced conformational changes of mitochondrial adenosine triphosphatase

✍ Scribed by Roux, Bernard; Fellous, Gilles; Godinot, Catherine

Book ID
127315239
Publisher
American Chemical Society
Year
1984
Tongue
English
Weight
461 KB
Volume
23
Category
Article
ISSN
0006-2960

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## Abstract Tightly bound adenine nucleotides are removed from multiple binding sites on beef heart mitochondrial ATPase (F~1~) by chromatography on columns of Sephadex equilibrated with 50% glycerol. Release of nucleotides from the enzyme is associated with large decreases in sedimentation velocit