Circadian Clocks: Methods and Protocols

✍ Scribed by Steven A. Brown

• Publisher: Springer US;Humana
• Year: 2021
• Tongue: English
• Leaves: 349
• Series: Methods in Molecular Biology 2130
• Edition: 1st ed.
• Category: Library

✦ Synopsis

This volume presents techniques used by researchers from all branches of biology to study daily changes at a molecular level in many physiological systems. The chapters are organized into three parts and cover topics such as measuring and modeling physiological and behavioral rhythms; genome-wide analyses in circadian biology, and imaging and manipulating brain clocks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Circadian Clocks: Methods and Protocols is a valuable tool for any researcher interested in learning more about this developing field.

✦ Table of Contents

Front Matter ....Pages i-xxii
Front Matter ....Pages 1-1
Real-Time In Vitro Fluorescence Anisotropy of the Cyanobacterial Circadian Clock (Joel Heisler, Archana Chavan, Yong-Gang Chang, Andy LiWang)....Pages 3-18
Inductively Coupled Plasma Mass Spectrometry for Elemental Analysis in Circadian Biology (Alessandra Stangherlin, Jason Day, John O’Neill)....Pages 19-27
The Assessment of Circadian Rhythms Within the Immune System (Chloé C. Nobis, Marc Cuesta, Jean-François Daudelin, Geneviève Dubeau Laramée, Diane B. Boivin, Nathalie Labrecque et al.)....Pages 29-51
Measuring Circadian Rhythms in Human Cells (Ngoc-Hien Du, Steven A. Brown)....Pages 53-67
Measuring the Effects of Circadian Rhythm-Related Manipulations on Depression-Like Behavior in Rodents: Forced Swim and Tail Suspension Tests (Chelsea A. Vadnie, Lauren M. DePoy, Colleen A. McClung)....Pages 69-78
Asking the Clock: How to Use Information from Questionnaires for Circadian Phenotyping (Céline Vetter, Eva C. Winnebeck, Till Roenneberg)....Pages 79-85
Simple Kinetic Models in Molecular Chronobiology (J. Patrick Pett, Pål O. Westermark, Hanspeter Herzel)....Pages 87-100
Front Matter ....Pages 101-101
Searching Novel Clock Genes Using RNAi-Based Screening (Bert Maier, Stephan Lorenzen, Anna-Marie Finger, Hanspeter Herzel, Achim Kramer)....Pages 103-114
Chromatin Immunoprecipitation (ChIP) from Mouse Liver Nuclei (Sara S. Fonseca Costa, Jürgen A. Ripperger)....Pages 115-125
Using GRO-Seq to Measure Circadian Transcription and Discover Circadian Enhancers (Bin Fang, Dongyin Guan, Mitchell A. Lazar)....Pages 127-148
Circadian Metabolomics from Breath (Steven A. Brown, Pablo Sinues)....Pages 149-156
A Lipidomics View of Circadian Biology (Rona Aviram, Chunyan Wang, Xianlin Han, Gad Asher)....Pages 157-168
Circadian Lipidomics: Analysis of Lipid Metabolites Around the Clock (Ursula Loizides-Mangold, Volodymyr Petrenko, Charna Dibner)....Pages 169-183
Phosphoproteome and Proteome Sample Preparation from Mouse Tissues for Circadian Analysis (Franziska Brüning, Sean J. Humphrey, Maria S. Robles)....Pages 185-193
Circadian Phosphorylation of CLOCK and BMAL1 (Hikari Yoshitane, Yoshitaka Fukada)....Pages 195-203
Front Matter ....Pages 205-205
Fluorescence Live Imaging of Drosophila Circadian Pacemaker Neurons (Virginie Sabado, Emi Nagoshi)....Pages 207-219
Monitoring Electrical Activity in Drosophila Circadian Output Neurons (Annika F. Barber, Amita Sehgal)....Pages 221-232
Light Input to the Mammalian Circadian Clock (Adam A. Dannerfjord, Laurence A. Brown, Russell G. Foster, Stuart N. Peirson)....Pages 233-247
Acute In Vivo Multielectrode Recordings from the Mouse Suprachiasmatic Nucleus (Joshua Mouland, Lauren Walmsley, Timothy M. Brown, Robert J. Lucas)....Pages 249-262
Perforated Multi-Electrode Array Recording in Hypothalamic Brain Slices (Mino D. C. Belle, Beatriz Baño-Otalora, Hugh D. Piggins)....Pages 263-285
Collection of Mouse Brain Slices for Bioluminescence Imaging of Circadian Clock Networks (Jennifer A. Evans, David K. Welsh, Alec J. Davidson)....Pages 287-294
Computational Analysis of PER2::LUC Imaging Data (Tanya L. Leise)....Pages 295-302
Electrophysiological Approaches to Studying the Suprachiasmatic Nucleus (Stephan Michel, Takahiro J. Nakamura, Johanna H. Meijer, Christopher S. Colwell)....Pages 303-324
Optogenetic Methods for the Study of Circadian Rhythms (Jeff R. Jones, Michael C. Tackenberg, Douglas G. McMahon)....Pages 325-336
Back Matter ....Pages 337-342

✦ Subjects

Life Sciences; Biochemistry, general; Animal Models