Cigarette smoke extract induced rat pulmonary artery smooth muscle cells proliferation via PKCα-mediated cyclin D1 expression
✍ Scribed by Da-xiong Zeng; Yong-jian Xu; Xian-sheng Liu; Ran Wang; Min Xiang
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 532 KB
- Volume
- 112
- Category
- Article
- ISSN
- 0730-2312
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✦ Synopsis
Cigarette smoke could induce pulmonary smooth muscle cells (PASMCs) proliferation. Although our previous study had implied the involvement of protein kinase Ca (PKCa), the molecular mechanism underlying PKCa pathway in this process is still unknown. In this study, rat PASMCs were stimulated by cigarette smoke extract (CSE) or PMA (a special activator to PKCa). Two percent CSE and PMA significantly enhanced cyclin D1 expression and cells proliferation. But cyclin D1-specific siRNA successfully inhibited DNA synthesis in CSE-treated or PMA-treated cells. On the other hand, PKCa-specific siRNA significantly suppressed cyclin D1 expression in CSE-treated cells. Moreover, PKCa-specific siRNA resulted in a cell-cycle arrest in G0/G1 and decreased cells number significantly. We conclude that CSE induced rat PASMCs proliferation at least partly via PKCa-mediated cyclin D1 expression.