✦ LIBER ✦

Cidofovir induces an increase in levels of low-risk and high-risk HPV E6

✍ Scribed by Adam J. Donne; Lynne Hampson; Xiaotong T. He; Michael P. Rothera; Jarrod J. Homer; Ian N. Hampson

Publisher: John Wiley and Sons
Year: 2009
Tongue: English
Weight: 335 KB
Volume: 31
Category: Article
ISSN: 1043-3074
DOI: 10.1002/hed.21043

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Background.

Cidofovir is a nucleoside analogue that is used off‐license to treat recurrent respiratory papillomatosis (RRP) caused by HPV6/11. However, the effect of this drug upon low‐risk HPV 6/11 gene expression is unknown.

Methods.

The expression of E6 was evaluated by RT‐PCR in HPV−ve C33A cervical carcinoma cells stably transfected with both low‐ and high‐risk HPV E6 cDNA's and in SiHa (HPV16+ve) cervical carcinoma cells after treatment with 2 doses and durations of exposure to cidofovir.

Results.

Compared to the vector only transcript, E6 RNA levels showed an 8‐fold increase in low‐risk and 20‐fold increase in high‐risk E6‐expressing cells. High‐risk E6 protein levels were also detected by Western blot in cidofovir‐treated C33A Type16 E6‐transfected cells.

Conclusion.

These data may indicate a potential rationale for increased risk of genetic instability and thus transformation due to drug‐induced increase in the level of E6. © 2009 Wiley Periodicals, Inc. Head Neck, 2009

📜 SIMILAR VOLUMES

Prevalence of low-risk and high-risk typ

Prevalence of low-risk and high-risk types of human papillomavirus and other risk factors for HPV infection in Germany within different age groups in women up to 30 years of age: An epidemiological observational study

✍ Thomas Iftner; Sonja Eberle; Angelika Iftner; Barbara Holz; Norbert Banik; Wim Q 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 255 KB

## Abstract Human papillomavirus (HPV) infection is frequent in young women and persistent infection may lead to cervical cancer. Therefore, vaccination against HPV is recommended for young women in the age group from 12–17 years in Germany. However, epidemiological data on the prevalence of HPV ty

Distribution of 37 mucosotropic HPV type

Distribution of 37 mucosotropic HPV types in women with cytologically normal cervical smears: The age-related patterns for high-risk and low-risk types

✍ Marcel V. Jacobs; Jan M.M. Walboomers; Peter J.F. Snijders; Feja J. Voorhorst; R 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 French ⚖ 202 KB 👁 1 views

High-risk HPV types in Tunisia. A pilot

High-risk HPV types in Tunisia. A pilot study reveals an unexpectedly high prevalence of types 58 and 82 and lack of HPV 18 among female prostitutes

✍ Federico De Marco; Fatma Houissa-Kchouk; Ridha Khelifa; Maria Luisa Marcante 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 122 KB

Women ≥30 years of age with low grade sq

Women ≥30 years of age with low grade squamous intraepithelial lesion (LSIL) have low positivity rates when cotested for high-risk human papillomavirus: Should we reconsider HPV triage for LSIL in older women?

✍ Michael J. Thrall; Debora A. Smith; Dina R. Mody 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 101 KB 👁 1 views

Both high and low levels of blood vitami

Both high and low levels of blood vitamin D are associated with a higher prostate cancer risk: A longitudinal, nested case-control study in the Nordic countries

✍ Pentti Tuohimaa; Leena Tenkanen; Merja Ahonen; Sonja Lumme; Egil Jellum; Göran H 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 French ⚖ 81 KB 👁 2 views

## Abstract Vitamin D inhibits the development and growth of prostate cancer cells. Epidemiologic results on serum vitamin D levels and prostate cancer risk have, however, been inconsistent. We conducted a longitudinal nested case‐control study on Nordic men (Norway, Finland and Sweden) using serum