Chronological changes in pyridoxine-5′-phosphate oxidase immunoreactivity in the seizure-sensitive gerbil hippocampus

✍ Scribed by Tae-Cheon Kang; Seung-Kook Park; In Koo Hwang; Sung-Jin An; Jae Hoon Bahn; A. Yeon Kim; Soo Young Choi; Oh-Shin Kwon; Nam-In Baek; Hyeon Yong Lee; Moo Ho Won

• Publisher: John Wiley and Sons
• Year: 2002
• Tongue: English
• Weight: 856 KB
• Volume: 68
• Category: Article
• ISSN: 0360-4012
• DOI: 10.1002/jnr.10280

✦ Synopsis

To identify the roles of pyridoxine-5'-phosphate (PNP) oxidase in epileptogenesis and the recovery mechanisms in spontaneous seizure, a chronological and comparative analysis of PNP oxidase expression was conducted. PNP oxidase immunoreactivity in a preseizure group of seizure-sensitive (SS) gerbils was detected more strongly than that in a seizure-resistant (SR) group. The density of PNP oxidase immunoreactivity in a 30 min postictal group was significantly lower than that in a preseizure group. In a 12 hr postictal group, PNP oxidase immunodensity had recovered to a preseizure level. The overexpression of PNP oxidase in the hippocampus of preseizure SS gerbils suggests that PNP or pyridoxal 5'-phosphate plays an important role in the modulation of glutamic acid decarboxylase activity and gamma-aminobutyric acid reuptake as mediated by membrane transporter via neurons. In addition, this change in the PNP oxidase immunoreactivity following seizure may be a compensatory response designed to reduce epileptic activity in this animal.