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Chromosome Segregation Controlled by External Mechanical Impulse in a Mammalian Cell

✍ Scribed by Itabashi, Takeshi; Ishiwata, Shin'ichi

Book ID: 122106752
Publisher: Biophysical Society
Year: 2012
Tongue: English
Weight: 34 KB
Volume: 102
Category: Article
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2011.11.1895

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## Abstract Pretreatment by hypothermic (25°C) cycling (PHC) of attached exponential‐phase V79 Chinese hamster cells by Method 4 (24 hr at 25°C + 1.5 hr at 37°C + 24 hr at 25°C + trypsin + 3 hr at 37°C) or by Method 3 (48 hr at 25°C + trypsin + 3 hr at 37°C) make mammalian V79 cells significantly m