Chromosome preparations from microplate cultures of man, dog, rat, and mouse

✍ Scribed by B. Jong; G. J. P. A. Anders; Ingrid H. Meer

• Publisher: Springer
• Year: 1976
• Tongue: English
• Weight: 209 KB
• Volume: 33
• Category: Article
• ISSN: 0340-6717
• DOI: 10.1007/bf00286855

✦ Synopsis

A simple method for making chromosomal preparations from 10(5) leukocytes from man, dog, mouse, and rat and from 0.01 ml total human and dog blood is developed. The leukocytes and the peripheral blood are cultured in Cooke microtiter plates in a culture volume of 0.1 ml. The culture medium is R.P.M.I. 1640 supplemented with serum and phytohemagglutinin. The culture time is 72 or 96 h.