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Chromosome preparations from hepatocytes and bone marrow of Chinese hamsters: A direct method

✍ Scribed by P. K. Srivastava; A. K. Shivastava; C. H. Bourgeois; F. V. Lucas

Publisher
Springer Netherlands
Year
1973
Tongue
English
Weight
358 KB
Volume
44
Category
Article
ISSN
0016-6707

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✦ Synopsis

This report describes a method for the direct preparation of chromosomes from the hepatocytes and bone marrow of the same Chinese hamster (Cricetulus griseus). The technique is a modification of that described by BECKER et al. (J. natl. Ca. Inst. 46 : 1261-69, 1971) for rat hepatocytes, with the following significant differences: (1) a less extensive partial hepatectomy is employed to initiate hepatocyte regeneration, (2) the use of a larger initial dose of colchicine (4 rag/K) 46-48 hours after hepatectomy instead of 1 mg/K 24 hours after hepatectomy, (3) the use of 0.075 M KC1 as hypotonic solution instead of fetal calf serum diluted 1:7 with distilled water and (4) flame or blaze drying of chromosome preparations instead of air drying. The combination of the above modifications gave abundant, clear and well-spread chromosomes.

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