Chromosome painting in acute monocytic leukemia

✍ Scribed by Dorra Cherif; Serge Romana; Hera Der-Sarkissian; Carol Jones; Roland Berger

• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 469 KB
• Volume: 6
• Category: Article
• ISSN: 1045-2257
• DOI: 10.1002/gcc.2870060207

✦ Synopsis

An Alu polymerase chain reaction (PCR) probe specific for chromosome I I prepared from the somatic cell hybrid J I was used to analyze karyotypes of eight patients with acute monocytic leukemia (AML-M5). Chromosome painting confirmed the t(9; I I) in one patient and a der(l)t(l;b)t(61 I) in another and allowed the identification of a complex rearrangement involving chromosomes 9, I I, and 17, previously classified as del( I l)(q23), in a third patient. An analysis of five patients with AML-M5 and a normal karyotype did not detect abnormalities of chromosome I I. The usefulness of chromosome painting combined with in situ hybridization with probes previously located on particular chromosomes is emphasized. Genes Chrom Cancer 6: 107-1 I2