Chromosome painting as a supplement to cytogenetic banding analysis in non-Hodgkin's lymphoma

✍ Scribed by Svetlana Bajalica; Anne-Grethe Sørensen; Niels Tinggaard Pedersen; Sverre Heim; Karen Brøndum-Nielsen

• Book ID: 102845475
• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 760 KB
• Volume: 7
• Category: Article
• ISSN: 1045-2257
• DOI: 10.1002/gcc.2870070409

✦ Synopsis

Chromosomal in situ suppression (CISS) hybridization with biotin labeled chromosome-specific libraries was performed on short-term cultures from five cases of non-Hodgkin's lymphoma (NHL). The painting analysis proceeded in three stages.

First-stage ClSS hybridization was done with libraries specific for chromosomes that seemed t o be lost or rearranged as judged by banding analysis. Second-stage ClSS included hybridization with probes specific for chromosomes that, because of banding pattern similarities, were considered to be likely candidates t o have contributed unidentified chromatin blocks in the abnormal karyotype. The third and final stage was a confirmation hybridization with a library specific for the chromosome that, at the stage two analysis, was found t o have donated the previously unknown chromosomal segment. The aberrant chromosomes were often more complex than the banding analysis had led us t o believe. Among the rearrangements whose nature was determined by ClSS hybridization were two add( I)(p36) which, in both cases, were shown t o be a der( I)t( 1;2)(p36;q31). This study illustrates the potential use of chromosome painting in resolving karyotypic uncertainties in NHL, and it shows that new cytogenetic subgroups may emerge when classical banding analysis is supplemented with fluorescence in situ hybridization techniques.