Chromosome numbers of ten-day fetal mouse cells

✍ Scribed by David A. Hungerford

• Publisher: John Wiley and Sons
• Year: 1955
• Tongue: English
• Weight: 688 KB
• Volume: 97
• Category: Article
• ISSN: 0362-2525
• DOI: 10.1002/jmor.1050970305

✦ Synopsis

Recent statements dealing with the chromosome complements of norntal mammalian somatic cells have disagreed regarding the frequency of aneuploidy and polyploidy present in such material. Therman and Timonen ('51) working with human uterine epithelium and human embryonic tissue, and Timorieri ( '50) with human uterine epithelium, found hypoploidy to be the T I I I ~, reporting for uterine epithelium (1000 cells) a bimodal distribution having a major peak at 30-25 chromosomes and a. secondary one at 45-50. This work has been criticized on technical grounds hy Walker and Boothroyd ('31) who, for mouse tissue and human cndonictrium, found "wide deviations from the diploid in the direction of hypoploidy when the tissue was prepared by the squash technique, but only slight deviations when the tissue was prepared by certain other methods."

Sachs ('53), using a E'eulgcn squasli tecliriique ('52), has counted chromosonies froni 50 cells each of humans, the white rat, and the short-tailed Iiank vole, Jlicrotus agrestis. Clainiing accuracy within 5% or better, he found in all cases no