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Chromosome banding, isoenzyme studies and determination of epstein-barr virus DNA content on human burkitt lymphoma/mouse hybrids

✍ Scribed by Jack Spira; Susanne Povey; Francis Wiener; George Klein; Maria Andersson-Anvret


Book ID
102278435
Publisher
John Wiley and Sons
Year
1977
Tongue
French
Weight
423 KB
Volume
20
Category
Article
ISSN
0020-7136

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✦ Synopsis


Abstract

Four independently fused hybrid clones derived from a cross between the mouse mammary carcinoma TA3Ha and the human Burkitt lymphoma line Daudi were tested for the EBV‐determined nuclear antigen (EBNA), EBV‐DNA and the presence of human chromosomes, in the course of serial propagation in vitro. EBNA and EBV‐DNA were lost in parallel with the loss of human chromosomes. It seems that the persistence of EB‐viral genetic information does not require the presence of a specific human chromosome(s) in this particular hybrid combination.