Chromosome aberrations in adenomas of the colon. Proof of trisomy 7 in tumor cells by combined interphase cytogenetics and immunocytochemistry
✍ Scribed by J. Herbergs; A. P. de Bruïne; P. T. J. Marx; M. I. J. Vallinga; R. W. Stockbrügger; F. C. S. Ramaekers; J. W. Arends; A. H. N. Hopman
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- French
- Weight
- 925 KB
- Volume
- 57
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
Thirty-five colon adenomas from 26 patients were analyzed with centromeric probes for chromosomes I, 7, 17, X and Y in order to study numerical aberrations, chromosome imbalances, aneuploidy and tetraploidization. The fluorescent in situ hybridization (FISH) technique was applied to single-cell suspensions and a combination of FISH and immunocytochemistry (ICC) was employed to identify the cell type under study. Trisomy of chromosome 7 was detected in 37% of the cases. In 7 out of I3 cases this aberration was combined with abnormalities of one or 2 of the other investigated chromosomes. N o correlation could