Chromosomal proteins of rat brain: Increased synthesis and affinity for DNA following a pulse of the carcinogen ethylnitrosourea in vivo
✍ Scribed by Leonard H. Augenlicht; Harald Biessmann; Manfred F. Rajewsky
- Publisher
- John Wiley and Sons
- Year
- 1975
- Tongue
- English
- Weight
- 581 KB
- Volume
- 86
- Category
- Article
- ISSN
- 0021-9541
No coin nor oath required. For personal study only.
✦ Synopsis
A single pulse of ethlnitrosourea (EtNU), administered to 10-day-old BD IX-rats, specifically results in a high incidence of neuroectodermal tumors in the central and peripheral nervous system. At five days after an EtNU-pulse, analyses of protein-DNA interactions were performed using chromatin dissociation and re-association experiments, following incorporation of radioactive leucine into brain chromosomal proteins (CP) during short-term suspension culture. In comparison with 15-day-old control animals, the brain cells of EtNU-treated rats exhibited (i) an increased rate of CP synthesis, and (ii) an increased affinity of the newly-synthesized CP for brain DNA of both control and EtNU-treated animals.