Chromosomal mobilization from a recA mutant of Pseudomonas putida

✍ Scribed by Chakrabarty, A. M. ;Gunsalus, I. C.

• Book ID: 104727267
• Publisher: Springer
• Year: 1979
• Tongue: English
• Weight: 355 KB
• Volume: 176
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00334307

✦ Synopsis

A methyl methane sulfonate sensitive mutant of P. putida strain PpG1 is also extremely sensitive to UV-rays, compared to parent wild type cells. This mutant behaves typically as recombination less (recA) mutants of Escherichia coli and Pseudomonas aeruginosa, since as a recipient, it exhibits extremely low frequency of recombination following conjugational, transductional, and transformational gene transfer. Sex factor plasmids such as K-XYL or TOL can mobilize chromosomal genes equally well both from recA+ and recA801 donor cells, suggesting that host recombination functions are not necessary for mobilization of chromosomal genes by such plasmids.