Chromosomal localization of the 5-HT1F receptor gene: No evidence for involvement in response to sumatriptan in migraine patients

✍ Scribed by VanDenBrink, Antoinette Maassen; Vergouwe, Monique N.; Ophoff, Roel A.; Naylor, Susan L.; Dauwerse, Hans G.; Saxena, Pramod R.; Ferrari, Michel D.; Frants, Rune R.

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 27 KB
• Volume: 77
• Category: Article
• ISSN: 0148-7299
• DOI: 10.1002/(sici)1096-8628(19980605)77:5<415::aid-ajmg12>3.0.co;2-l

✦ Synopsis

The 5-HT 1F receptor, which is present in both human vascular and neuronal tissue, may mediate the therapeutic effect and/or side-effects of sumatriptan. We investigated the chromosomal localization of the 5-HT 1F receptor gene and the relation between eventually existing polymorphisms and the clinical response to sumatriptan in migraine patients. The 5-HT 1F receptor gene was localized using a monochromosomal mapping panel, followed by a radiationreduced hybrid mapping and fluorescent in situ hybridization. The results of these techniques show that the 5-HT 1F receptor gene is localized at 3p12. We investigated the presence of polymorphisms by single strand conformation polymorphism analysis in 14 migraine patients who consistently responded well to sumatriptan, 12 patients who consistently experienced recurrence of the headache after initial relief, 12 patients with no response to sumatriptan, and in 13 patients who consistently experienced chest symptoms after use of sumatriptan. No polymorphisms were detected in any of the patients. We therefore conclude that genetic diversity of the 5-HT 1F receptor gene is most probably not responsible for the variable clinical response to sumatriptan.