A mouse cell line (LP1-1) was established from the murine L cells deficient in thymidine kinase (L-M(TK-] by prolonged selective culture on the hypoxanthine-aminopterine-thymidine (HAT) medium following transfection with the thymidine kinase gene of herpes simplex virus type-I (HSVTK). Southern blot
Chromosomal gene transfer of human cytosol thymidine kinase into mouse cells
✍ Scribed by Willecke, Klaus ;Mierau, Rudolf ;Krüger, Almut ;Lange, Rita
- Publisher
- Springer
- Year
- 1978
- Tongue
- English
- Weight
- 857 KB
- Volume
- 161
- Category
- Article
- ISSN
- 0026-8925
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## Abstract Isozyme analyses have been carried out to investigate the sites of integration of the herpes simplex type 2 (HSV‐2) thymidine kinase (TK) gene in biochemically transformed human [HeLa(BU25)/HSV‐2–6 Cl 4] cells. Extracts were prepared from He La (BU25)/HSV‐2–6 Cl 4 cells and from human—m
lacZ gene driven by the b-actin -based promoter. Ex-SEE EDITORIAL ON PAGE 1696. pression of the HSVtk gene by adenovirus from AFP promoter/enhancer (AdAFPtk) induced the cells sensitive to ganciclovir (GCV) in the AFP-producing cell We have developed a recombinant replication-deline efficiently, but