Chromosomal changes in three cell strains of the tasmanian rat-kangaroo,Potorous tridactylis, culturedin vitro(Marsupialia)

✍ Scribed by K. Heyman-Zandstra; H. Steenis

• Publisher: Springer Netherlands
• Year: 1973
• Tongue: English
• Weight: 426 KB
• Volume: 44
• Category: Article
• ISSN: 0016-6707
• DOI: 10.1007/bf00155958

✦ Synopsis

The chromosomal changes occurring in three strains of cells from Potorous tridactylis, one derived from testis and two of kidney tissue, were followed during the in vitro life of the strains.

One kidney cell strain was a slow growing one and died after 23 passages showing aneuploidy with very aberrant metaphases. The strain derived from testis showed aneuploidy after a period of growth retardation, about 50% of the aneuploid cells having 18, 19 or 20 chromosomes. In these cells the chromosomes 1, 2 and 4 were always present in triplicate and the cells always had two X-chromosomes. The second kidney strain showed aneuploidy after a period of growth retardation, cells with 22 and 23 chromosomes being the most frequent ones, but in different proportions. As the number of aneuploid cells gradually decreased, diploid cells appeared in the population. Their number also decreased and a new population of aneuploid cells arose, having 23 chromosomes, missing one chromosome nr. 5 from the tetraploid complement. Then again the cell strain returned to diploidy but as the frequency of these diploid cells decreased, the strain died ont.