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Chromophore-assisted laser inactivation (CALI) to validate drug targets and pharmacogenomic markers

✍ Scribed by Leodevico L. Ilag; Jocelyn H. Ng; Daniel G. Jay


Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
141 KB
Volume
49
Category
Article
ISSN
0272-4391

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✦ Synopsis


Functional proteomics directly addresses the role of proteins in cellular processes and thereby provides a basis for target validation for the development of therapeutics against particular diseases. Validated protein targets may serve as ideal pharmacogenomics markers. Chromophore-assisted laser inactivation (CALI) is a powerful tool for functional proteomics via protein inactivation with advantages of high spatial and temporal resolution. It also provides a complementary alternative to other inactivation strategies. It is hoped that future applications of CALI will accelerate the drug discovery process. In addition, CALI may be used to address problems associated with elucidating the function of multiple interacting genes through the use of multiple protein inactivations to show epistasis of complex cellular pathways.