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Chromogenic analogues of penicillin dihydroF and penicillin K for the continuous spectrophotometric determination of aliphatic penicillin acylase activity

✍ Scribed by Miguel Arroyo; Raquel Torres-Guzmán; Jesús Torres-Bacete; Isabel de la Mata; María Pilar Castillón; Carmen Acebal

Book ID
110345565
Publisher
Springer Netherlands
Year
2002
Tongue
English
Weight
58 KB
Volume
24
Category
Article
ISSN
0141-5492

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✍ Luuk M. van Langen; Michiel H. A. Janssen; Natasja H. P. Oosthoek; Sílvia R. M. 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 92 KB 👁 2 views

## Abstract Native and immobilized preparations of penicillin acylase from __Escherichia coli__ and __Alcaligenes faecalis__ were studied using an active site titration technique. Knowledge of the number of active sites allowed the calculation of the average turnover rate of the enzyme in the vario