Chromatography of vitamin A compounds on silicic acid columns

✍ Scribed by M. Zile; H.F. DeLuca

• Book ID: 102981806
• Publisher: Elsevier Science
• Year: 1968
• Tongue: English
• Weight: 574 KB
• Volume: 25
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(68)90105-x

✦ Synopsis

In work with vitamin A compounds it is often necessary to employ chromatogruphic methods for their separation or purification. The most commonly used adsorbent for column chromatography of vitamin A compounds is alumina. Silicic acid, although adopted universally for the chromatography of most lipids, has been used only to a very limited extent in the vitamin A field (l-4). In our laboratory, however, we have found this adsorbent to be very useful for the separation of various vitamin A compounds from each other and also from some lipid-soluble vitamins (5-7). Our experiences with silicic acid are recorded in t'his comnnniicat~ion.

MATERIAIJS AND METHODS

Chemicals

iZll-trails-retinoic acid, retinol, retinene, retinyl acetate, and retinyl palmitate were obtained from Distillation Products Industries, Rochester, N. Y. 9,13-Di-cis-retinoic acid, 13-c%-and 9-cis-retinols were gifts from the same company.