Chromatography of some cystine peptides and formation of mixed disulfides

✍ Scribed by James J. Richter; Arthur Wainer

• Book ID: 104147318
• Publisher: Elsevier Science
• Year: 1968
• Tongue: English
• Weight: 915 KB
• Volume: 37
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)99070-3

✦ Synopsis

Cystine peptides were prepared in the sulfhydryl form by reducing the disulfide bonds with dithiothreitol. The reduced peptides were separated from dithiothreitol on a small column of Dowex 5oW. X4.in the H+ form. The sulfhydryl peptides were mixed. together and' allowed to aoxidize .eompletely. The. oxidized. products' were analyzed in. the chromatographio system. The formation 'of symmetrical 'and mixed disulfides from the sulfhydryl compounds appeared to occur randomly. 1 '. two resulting1 thiol compounds. Further study of mixed disulfides has been limited by the techniques available.:This pap.er reports the use .of ion-exchange: chromatography. to study disulfide formation.'from cysteine-containing peptides; ' ;

:. 1 : : -1 !. ' ' -The. work consisted, of .th&e:.mairr problems: First; ! aohromatograpliic system for analy!ing the amounts .of 'peptides had to he established. Because tl~e~comn~ercially