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Chromatographic separation of the C(1), C(1a), and C(2) components of gentamicin and the assessment of their individual binding to serum proteins

✍ Scribed by William C. Griffiths; James F. Belliveau; Charlene L. Bensted; Steven G. Calabresi; Israel Diamond

Publisher
John Wiley and Sons
Year
1984
Tongue
English
Weight
256 KB
Volume
73
Category
Article
ISSN
0022-3549

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✦ Synopsis

0 [3H]

gentamicin and [14C]gentamicin samples were purified by Sephadex column chromatography and separated by an HPLC technique into the three major, medicinally active gentamicin components. These separated components were used in equilibrium dialysis studics to dctcrmine their percent binding to serum proteins. The bindings of the componcnts wcrc inversely relatcd to concentrations of ionized calcium and magnesium. When dialyzed against a buffer containing physiological concentrations of the divalent cations, the bindingof theC(l)componentwas2.2 f I.O%.the bindingoftheC(1a) component was 1.2 f 1.9%, and the binding of the C(2) component was 5.0 f 2.0%. The percent bindings are not identical and, due to their low values, probably have negligible clinical significance. The radioactive composition and purity of the 3H-and I4C-labeled gentamicin samples differed and thcsc may be important factors in thc variance of reported gentamicin bindings.

Keyphrases 0 Gentarnicin-component separation serum protein binding. radiolabels, HPLC 0 Serum protein binding-radiolabeled gentamicin, component separation, HPLC

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