✦ LIBER ✦

Chromatographic separation and identification of nucleotides from animal tissues and yeast cells on a polyethyleneimine cellulose column

✍ Scribed by K.-H. Pflüger

Publisher: Elsevier Science
Year: 1977
Tongue: English
Weight: 382 KB
Volume: 81
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(77)90606-6

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✦ Synopsis

A procedure has been developed for the separation and identification of nucleotides by anion-exchange chromatography. The method includes column chromatography on PEI-cellulose with an unbuffered exponential salt gradient. This easily handled procedure gives a high degree of resolution and reproducibility and demands no personal attendance during elution. Application of the method is demonstrated with a mouse liver extract and extracts of yeast cells (Saccltarowtyces cere~'i.viae).